Effect of Euphorbia cyparissias ointments on acanthosis.

BACKGROUND A pharmaco-chemical investigation of the Euphorbia cyparissias plant was justified by its known multiple therapeutic valences. Numerous components from extracts and latex of Euphorbiacae were identified, revealing a large plant family with a polyvalent therapeutic activity. MATERIALS AND METHODS The aim of the study was to assess the skin tolerance level to irritation on different testing concentrations, of Euphorbia cyparissias extracts and ointments. Study was accomplished in rats and dogs, with the identification of all possible skin injuries and histological changes, after a simple patch test methodology. RESULTS Ointment dermatological testing on rats, proved to be bearable on epilated skin at concentrations of 1, 2 and 5%. Ointments and mother tincture with higher concentrations (10% and 20%), led to irritation and cutis damages, and this was revealed through histology. CONCLUSION Ointment tested on dog's skin was tolerable for epilated skin to concentrations of 1, 2 and/or 5%, additional testing on human volunteers confirmed the same situation.